Low Doses of Gamma Irradiation Stimulate Synthesis of Bioactive Compounds with Antioxidant Activity in Fomes fomentarius Living Mycelium

Applied Sciences

Alina-Maria Petrache¹  DanielConstantin Neguț²  CristinaFlorentina Pelcaru²  Mihaela Ene² 

[1] Department of Proteomics and Metabolomics, Research Center for Advanced Medicine—MedFUTURE, “Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-Napoca, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania;“Horia Hulubei” National Institute for Physics and Nuclear Engineering (IFIN-HH), Reactorului Street 30, 077125 Măgurele, Romania;

关键词: mycelium;    antioxidant activity;    gamma irradiation;    Fomes fomentarius;    mushroom;    DPPH;   

DOI  :  10.3390/app11094236

来源: DOAJ

【 摘 要 】

Environmental changes generate free radicals and reactive oxygen species (ROS), resulting in abiotic stress in plants and fungi. Gamma ionizing radiation generates a significant amount of free radicals and ROS, thereby simulating natural environmental stressors. We used a ⁶⁰Co source of radiation to experimentally induce oxidative stress in living mycelium mass of the medicinal fungus Fomes fomentarius, in order to obtain a late response of stress tolerance by means of bioactive compounds synthesis. We measured the response at 24, 48, and 72 h after the irradiation. The highest improvement was found 24 h after exposure for antioxidant activity and for total phenolic compounds of methanolic extract, with a 1.89- and 1.64-fold increase, respectively. The total flavonoids in methanolic extract increased 1.68 times after 48 h from treatment and presented a more stable raising in the assessed time-lapse. For the three analyzed parameters, 300 Gy was the optimum absorbed dose to trigger a beneficial response, with potentially applications in pharmaceutics and nutraceutics. Gamma irradiation can be used as a biotechnological tool to direct the secondary metabolites synthesis upregulation in medicinal mushroom living mycelium.

【 授权许可】

Unknown