UTMD promoted local delivery of miR-34a-mimic for ovarian cancer therapy

Drug Delivery

Jinghui Fang¹  Jia Zhou²  Meng Du³  Yue Li³  Zhiyi Chen³ 

[1] Laboratory of Ultrasound Molecular Imaging, The Third Affiliated Hospital of Guangzhou Medical University;The First Affiliated Hospital, Department of Ultrasound Medicine, Hengyang Medical School, University of South China;The First Affiliated Hospital, Medical Imaging Centre, Hengyang Medical School, University of South China;

关键词: ultrasound;    microbubbles;    multi-parameters;    mir-34a mimic;    cancer therapy;   

DOI  :  10.1080/10717544.2021.1955041

来源: DOAJ

【 摘 要 】

MicroRNA-mediated gene therapy is emerging as a promising method for the treatment of ovarian cancer, but the development of miRNA mimic delivery vectors is still in its infancy, where the safety and efficacy of miR-34a-mimic remain unknown. Ultrasound-targeted microbubble destruction (UTMD) can be an effective and minimally invasive tool for the delivery of miR-34a-mimic in vitro and in vivo. Here, we describe a high-efficiency gene delivery strategy by using miR-34a-mimic loaded folate modified microbubbles (miR-34a-FM) with a portable ultrasonic irradiation system. Ultrasonic parameters, including acoustic intensity (AI), exposure time (ET) and duty cycle (DC), were optimized and the optimal acoustic condition (1.0 W/cm2, 20 s, and 15% DC) was used to deliver miRNA-34a into cells in vitro. MiR-34a mimic was successfully introduced into the cytoplasm and was found to inhibit proliferation and induce apoptosis of SK-OV-3 cells. Next, miR-34a-mimic was delivered to tumor tissue via UTMD, inhibiting tumor growth and prolonging the survival time of mice. In summary, UTMD-mediated miR-34a-mimic delivery has potential application in the clinical treatment of ovarian cancer.

【 授权许可】

Unknown