Combined Buckwheat d-Fagomine and Fish Omega-3 PUFAs Stabilize the Populations of Gut Prevotella and Bacteroides While Reducing Weight Gain in Rats

Nutrients

Susana Amézqueta¹  Marta Romeu²  Bernat Miralles-Pérez²  JosepLluís Torres³  Roser Marín-Valls³  Mercè Hereu³  Sara Ramos-Romero³  Lucía Méndez⁴  Isabel Medina⁴ 

[1] Departament d’Enginyeria Química i Química Analítica and Institut de Biomedicina (IBUB), Universitat de Barcelona, E-08028 Barcelona, Spain;Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, E-43201 Reus, Spain;Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), E-08034 Barcelona, Spain;Instituto de Investigaciones Marinas (IIM-CSIC), E-36208 Vigo, Spain;

关键词: weight;    gut microbiota;    prevotella;    bacteroides;    d-fagomine;    iminosugar;    iminocyclitol;    ω-3 pufas;   

DOI  :  10.3390/nu11112606

来源: DOAJ

【 摘 要 】

Some functional food components may help maintain homeostasis by promoting balanced gut microbiota. Here, we explore the possible complementary effects of d-fagomine and ω-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA 1:1) on putatively beneficial gut bacterial strains. Male Sprague−Dawley rats were supplemented with d-fagomine, ω-3 PUFAs, or both, for 23 weeks. Bacterial subgroups were evaluated in fecal DNA by quantitative real-time polymerase chain reaction (qRT-PCR) and short-chain fatty acids were determined by gas chromatography. We found that the populations of the genus Prevotella remained stable over time in animals supplemented with d-fagomine, independently of ω-3 PUFA supplementation. Animals in these groups gained less weight than controls and rats given only ω-3 PUFAs. d-Fagomine supplementation together with ω-3 PUFAs maintained the relative populations of Bacteroides. ω-3 PUFAs alone or combined with d-fagomine reduced the amount of acetic acid and total short-chain fatty acids in feces. The plasma levels of pro-inflammatory arachidonic acid derived metabolites, triglycerides and cholesterol were lower in both groups supplemented with ω-3 PUFAs. The d-fagomine and ω-3 PUFAs combination provided the functional benefits of each supplement. Notably, it helped stabilize populations of Prevotella in the rat intestinal tract while reducing weight gain and providing the anti-inflammatory and cardiovascular benefits of ω-3 PUFAs.

【 授权许可】

Unknown