Thermophilic Anaerobic Digestion of Second Cheese Whey: Microbial Community Response to H2 Addition in a Partially Immobilized Anaerobic Hybrid Reactor

Processes

Antonella Marone¹  Antonella Signorini²  Valentina Mazzurco Miritana²  Giuseppe Lembo²  Silvia Rosa²  Giulia Massini²  Massimiliano Fenice³ 

[1] Department of Energy Efficiency Unit, Casaccia Research Center, ENEA-Italian Agency for New Technologies, Energy and Sustainable Development, Via Anguillarese 301, 00123 Rome, Italy;Department of Energy Technologies and Renewable Source, Casaccia Research Center, ENEA-Italian Agency for New Technologies, Energy and Sustainable Development, Via Anguillarese 301, 00123 Rome, Italy;Ecological and Biological Sciences Department, University of Tuscia, 01100 Viterbo, Italy;

关键词: cheese whey;    anaerobic hybrid reactor;    thermophilic anaerobic digestion;    in situ hydrogen addition;    microbial community;   

DOI  :  10.3390/pr9010043

来源: DOAJ

【 摘 要 】

In this study, we investigated thermophilic (55 °C) anaerobic digestion (AD) performance and microbial community structure, before and after hydrogen addition, in a novel hybrid gas-stirred tank reactor (GSTR) implemented with a partial immobilization of the microbial community and fed with second cheese whey (SCW). The results showed that H₂ addition led to a 25% increase in the methane production rate and to a decrease of 13% in the CH₄ concentration as compared with the control. The recovery of methane content (56%) was reached by decreasing the H₂ flow rate. The microbial community investigations were performed on effluent (EF) and on interstitial matrix (IM) inside the immobilized area. Before H₂ addition, the Anaerobaculaceae (42%) and Lachnospiraceae (27%) families dominated among bacteria in the effluent, and the Thermodesulfobiaceae (32%) and Lachnospiraceae (30%) familiesdominated in the interstitial matrix. After H₂ addition, microbial abundance showed an increase inthe bacteria and archaea communities in the interstitial matrix. The Thermodesulfobiaceae family (29%)remained dominant in the interstitial matrix, suggesting its crucial role in the immobilized community and the SHA-31 family was enriched in both the effluent (36%) and the interstitial matrix (15%). The predominance of archaea Methanothermobacter thermoautrophicus indicated that CH₄ was produced almost exclusively by the hydrogenotrophic pathway.

【 授权许可】

Unknown