Creation of an ex-vivo bovine kidney flow model for testing embolic agents: work in progress

CVIR Endovascular

Charles Hyman¹  John Walker¹  Matthew Parker¹  Barrett O’Donnell¹  Ho-Young Song¹  Carlos Ortiz¹  Jorge Lopera²  Luis Garza²  Ryan Bitar² 

[1] Department of Radiology, University of Texas Health Science Center;Long School of Medicine, University of Texas Health Science Center;

关键词: Flow model;    Perfusion;    Bovine kidney;    Embolization;    Fluoroscopy;   

DOI  :  10.1186/s42155-021-00210-0

来源: DOAJ

【 摘 要 】

Abstract Objectives To develop an ex- vivo perfusion flow model using a bovine kidney for future testing of embolic agents in an inexpensive and easy way. Methods Six bovine adult kidneys were used for this study. Kidneys were cannulated and perfused via a roller pump. Three embolic agents, coils, Gelfoam, and a glue mixture of Histoacryl + Lipiodol, were deployed by targeting three secondary segmental arteries per kidney via a 5Fr catheter under fluoroscopic guidance. Cannulation time, success rate of segmental artery selection and embolic agent deployment, total operational time, and fluoroscopy dose were recorded. Results Average kidney weight was 0.752 +/− 0.094 kg. All six bovine kidneys were successfully cannulated in 21.6 min +/− 3.0 min. Deployment of coils and glue was achieved in every case (12/12); however, Gelfoam injection was not successful in one instance (5/6, 83%). Coil deployment demonstrated no embolic effect while Gelfoam and glue injections demonstrated decreased distal contrast filling post-embolization. Mean dose area product was 12.9 ± 1.8 Gy·cm2, fluoroscopy time was 10 ± 4 min and operational time was 27 ± 8 min. Conclusions We describe the creation of an ex vivo bovine kidney flow model for the preclinical evaluation of different embolic materials. The flow model can be modified to provide extensive bench testing and it is a promising tool for hands -on training in basic and advanced embolization techniques .

【 授权许可】

Unknown