Parameter Reliability and Understanding Enzyme Function

Molecules

Keith F. Tipton¹  Andrew G. McDonald¹ 

[1] School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, D02 PN40 Dublin, Ireland;

关键词: enzyme kinetics;    Michaelis constant;    maximal velocity;    substrate competition;    enzyme inhibition;    cooperativity;   

DOI  :  10.3390/molecules27010263

来源: DOAJ

【 摘 要 】

Knowledge of the Michaelis–Menten parameters and their meaning in different circumstances is an essential prerequisite to understanding enzyme function and behaviour. The published literature contains an abundance of values reported for many enzymes. The problem concerns assessing the appropriateness and validity of such material for the purpose to which it is to be applied. This review considers the evaluation of such data with particular emphasis on the assessment of its fitness for purpose.

【 授权许可】

Unknown