Potent anti-tumor immunostimulatory biocompatible nanohydrogel made from DNA

Nanoscale Research Letters

Anyue Xia¹  Peifeng Liu¹  Xue Zhao¹  Ding Ma¹  Xiaozhen He¹  Jiana Jiang¹  Xianming Kong¹  Hanbing Zou¹  Yuexia Xie¹  Qianyun Tang² 

[1] Central Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong University;State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University;

关键词: CpG;    Nanohydrogel;    Immunotherapy;    Macrophage;    Glioma;   

DOI  :  10.1186/s11671-019-3032-9

来源: DOAJ

【 摘 要 】

Abstract Unmethylated CpG oligodeoxynucleotides are potent immunostimulatory motifs in activating both innate and acquired immune system by inducing Th1 type antigen-specific T cell responses, but their instability in serum greatly influences their immunostimulant efficiency. Here, we constructed a novel immuno-DNA nanohydrogels consisting of tandem repeat sequences of CpG units named CpG-MCA nanohydrogels through multi-primed chain amplification. CpG-MCA nanohydrogels were proved to resist degradation and increase the proliferation and migration of murine macrophage-like RAW264.7 cells. Furthermore, CpG-MCA nanohydrogels effectively induced high expression of tumor necrosis factor-α and interleukin-6, and remarkably inhibited the proliferation of U251 cells, suggesting that CpG-MCA nanohydrogels are expected to be employed as the potent anti-cancer immunostimulant.

【 授权许可】

Unknown