Validation of baboon pluripotent cells as a model for translational stem cell research

Stem Cell Research

John R. McCarrey¹  Shital Chaudhari²  Christopher S. Navara²  Megan A. Mahlke²  Keren Cheng²  Bo Li² 

[1] UTSA Stem Cell Core Lab, University of Texas at San Antonio, San Antonio, TX 78249, USA;Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USA;

关键词: Nonhuman primate;    Model system;    Embryonic stem cells;    Induced pluripotent stem cells;    Preclinical validation of stem cell therapies;   

DOI  :  

来源: DOAJ

【 摘 要 】

Translation of stem cell therapies to the clinic will be most successful following optimization of efficacy and safety in appropriate preclinical model systems. Among available models, nonhuman primates (NHPs) provide the most accurate recapitulation of human anatomy, physiology, genetics and epigenetics. Here, we show that baboon pluripotent cells (PSCs) recapitulate key molecular features of human PSCs with greater accuracy than that found in PSCs from non-primate species such as mice. Specifically, baboon and human PSCs exhibit greater conservation of gene expression patterns, higher sequence and structural homology among pluripotency factors, more equivalent genome-wide patterns of histone and DNA methylation modifications, and similar maintenance of bivalent programming of developmental genes than that found between human and non-primate PSCs.

【 授权许可】

Unknown