A programmable culture platform for hydrostatic stimulation and in situ pH sensing of lung cancer cells with organic electrochemical transistors

Micro and Nano Engineering

Francesca Frascella¹  Matteo Parmeggiani²  Simone Luigi Marasso³  Matteo Cocuzza³  Désirée Baruffaldi⁴  Simona Villata⁴  Cacocciola Nicolò⁴  Giancarlo Canavese⁴  Candido Fabrizio Pirri⁴ 

[1]CNR-IMEM, Parco Area delle Scienze 37a, 43124 Parma, Italy

[2]Corresponding author.

[3]PolitoBIOMed Lab, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Turin, Italy

[4]DISAT - Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy

关键词: Lung-on-a-chip;    Bioreactor;    Lung cancer;    Precision medicine;    Organic electrochemical transistors;    pH-sensing;   

DOI  :  

来源: DOAJ

【 摘 要 】

In this work, we present a programmable dynamic cell culture stimulation platform designed to work in a CO2 incubator, compatible with a standard multi-well plate. An epithelial lung cell culture can be mechanically stimulated with a cyclic increase of the hydrostatic pressure inside the culture chamber in the range 1–12 kPa, trying to mimic the human respiration rate. An in situ live pH-monitoring system based on PEDOT:PSS Organic ElectroChemical Transistors was also designed, fabricated and characterized, in order to merge both the stimulated cell culture and the pH sensing in a compact platform.

【 授权许可】

Unknown