Induction of cell cycle arrest via the p21, p27–cyclin E,A/Cdk2 pathway in SMMC-7721 hepatoma cells by clioquinol

Acta Pharmaceutica

Wang Lianqiu¹  Huang Zhiwei¹  Chen Lifeng¹  Shi Ping²  Zhang Yifei² 

[1] Key Lab of Eco-Textile (Ministry of Education), College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology Shanghai 200237, China;

关键词: clioquinol;    human smmc-7721 hepatoma cells;    cell cycle s-phase arrest;    cyclin e/a-cdk2;   

DOI  :  10.1515/acph-2015-0034

来源: DOAJ

【 摘 要 】

Clioquinol has been shown to have anticancer activity in several carcinoma cells. In this study, we preliminarily examined the effect of clioquinol in human SMMC-7721 hepatoma and QSG-7701 normal hepatic cells. Our results indicated that clioquinol did not significantly affect survival of QSG-7701 cells, whereas it reduced cell viability in a concentration- and time-dependent manner in SMMC-7721 cells. Clioquinol did not trigger autophagy and apoptosis, while it induced cell cycle arrest in the S-phase in SMMC- 7721 cells. Additionally, down-regulation of cyclin D1, A2, E1, Cdk2 and up-regulation of p21, p27 were detected after the treatment with clioquinol. The results demonstrated for the first time that clioquinol suppressed cell cycle progression in the S-phase in SMMC-7721 cells via the p21, p27-cyclin E,A/Cdk2 pathway. This suggests that clioquinol may have a therapeutic potential as an anticancer drug for certain malignances.

【 授权许可】

Unknown