MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE

Scientific Papers Animal Science and Biotechnologies

A. VLAIC¹  CLAUDIA TEREZIA SOCOL¹  VIORICA COSIER¹ 

[1] University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Husbandry and Biotechnology, Cluj-Napoca;

关键词: ligation reaction;    bacterial transformation;    recombinants selection;   

DOI  :  

来源: DOAJ

【 摘 要 】

An efficient bacterial transformation system suitable for cloning the coding sequence of the ovine leptin gene in E. coli DH5α host cells using the pGEMT easy vector it is described in this paper. The necessity of producing leptin is based on the fact that the role of this molecule in the animal and human organism is still unknown, leptin not existing as commercial product on the Romanian market. The results obtained in the bacterial transformation, cloning, recombinant clones selection, control of the insertion experiments and DNA computational analysis represent the first steps in further genetic engineering experiments such as production of DNA libraries, DNA sequencing, protein expression, etc., for a further contribution in elucidating the role of leptin in the animal and human organism.

【 授权许可】

Unknown