Epigenetic age-predictor for mice based on three CpG sites

eLife

Vadim Sakk¹  Laura Yndriago¹  Monika Eipel¹  Yang Han²  Julia Franzen²  Bertien Dethmers-Ausema²  Ander Izeta³  Gerald de Haan⁴  Hartmut Geiger⁵  Wolfgang Wagner⁵ 

[1] Institute for Biomedical Engineering – Cell Biology, University Hospital RWTH Aachen, Aachen, Germany;Helmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical School, Aachen, Germany;Institute of Molecular Medicine, Ulm University, Ulm, Germany;Laboratory of Ageing Biology and Stem Cells, European Research Institute for the Biology of Ageing, University Medical Center Groningen, Groningen, Netherlands;Tissue Engineering Laboratory, Instituto Biodonostia, San Sebastian, Spain;

关键词: aging;    age;    clock;    DNA methylation;    predictor;    signature;   

DOI  :  10.7554/eLife.37462

来源: DOAJ

【 摘 要 】

Epigenetic clocks for mice were generated based on deep-sequencing analysis of the methylome. Here, we demonstrate that site-specific analysis of DNA methylation levels by pyrosequencing at only three CG dinucleotides (CpGs) in the genes Prima1, Hsf4, and Kcns1 facilitates precise estimation of chronological age in murine blood samples, too. DBA/2 mice revealed accelerated epigenetic aging as compared to C57BL6 mice, which is in line with their shorter life-expectancy. The three-CpG-predictor provides a simple and cost-effective biomarker to determine biological age in large intervention studies with mice.

【 授权许可】

Unknown