Zingerone Modulates Neuronal Voltage-Gated Na+ and L-Type Ca2+ Currents

International Journal of Molecular Sciences

Ming-Chi Lai¹  Chin-Wei Huang²  Sheng-Nan Wu³ 

[1] Chi-Mei Medical Center, Department of Pediatrics, Tainan 71004, Taiwan;Department of Neurology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan;Department of Physiology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan;

关键词: zingerone (gingerone;    vanillylacetone);    voltage-gated Na⁺ current;    persistent Na⁺ current;    L-type Ca²⁺ current;    hysteresis;   

DOI  :  10.3390/ijms23063123

来源: DOAJ

【 摘 要 】

Zingerone (ZO), a nontoxic methoxyphenol, has been demonstrated to exert various important biological effects. However, its action on varying types of ionic currents and how they concert in neuronal cells remain incompletely understood. With the aid of patch clamp technology, we investigated the effects of ZO on the amplitude, gating, and hysteresis of plasmalemmal ionic currents from both pituitary tumor (GH₃) cells and hippocampal (mHippoE-14) neurons. The exposure of the GH₃ cells to ZO differentially diminished the peak and late components of the I_Na. Using a double ramp pulse, the amplitude of the I_Na(P) was measured, and the appearance of a hysteresis loop was observed. Moreover, ZO reversed the tefluthrin-mediated augmentation of the hysteretic strength of the I_Na(P) and led to a reduction in the I_Ca,L. As a double ramp pulse was applied, two types of voltage-dependent hysteresis loops were identified in the I_Ca,L, and the replacement with BaCl₂-attenuated hysteresis of the I_Ca,L enhanced the I_Ca,L amplitude along with the current amplitude (i.e., the I_Ba). The hysteretic magnitude of the I_Ca,L activated by the double pulse was attenuated by ZO. The peak and late I_Na in the hippocampal mHippoE-14 neurons was also differentially inhibited by ZO. In addition to acting on the production of reactive oxygen species, ZO produced effects on multiple ionic currents demonstrated herein that, considered together, may significantly impact the functional activities of neuronal cells.

【 授权许可】

Unknown