Modulation and Protection Effects of Antioxidant Compounds against Oxidant Induced Developmental Toxicity in Zebrafish

Antioxidants

Jesus Gomez-Catalan¹  Elisabet Teixido¹  JuanMaria Llobet¹  Nuria Boix¹  Ester Pique¹ 

[1] Toxicology Unit, Pharmacology, Toxicology and Therapeutical Chemistry Department, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n 08028 Barcelona, Spain;

关键词: oxidative stress;    zebrafish embryo;    in vivo model;    antioxidant effect;   

DOI  :  10.3390/antiox9080721

来源: DOAJ

【 摘 要 】

The antioxidant effect of compounds is regularly evaluated by in vitro assays that do not have the capability to predict in vivo protective activity or to determine their underlying mechanisms of action. The aim of this study was to develop an experimental system to evaluate the in vivo protective effects of different antioxidant compounds, based on the zebrafish embryo test. Zebrafish embryos were exposed to tert-butyl hydroperoxide (tBOOH), tetrachlorohydroquinone (TCHQ) and lipopolysaccharides from Escherichia coli (LPS), chemicals that are known inducers of oxidative stress in zebrafish. The developmental toxic effects (lethality or dysmorphogenesis) induced by these chemicals were modulated with n-acetyl l-cysteine and Nω-nitro l-arginine methyl ester hydrochloride, dimethyl maleate and dl-buthionine sulfoximine in order to validate the oxidant mechanism of oxidative stress inducers. The oxidant effects of tBOOH, TCHQ, and LPS were confirmed by the determination of significant differences in the comparison between the concentration–response curves of the oxidative stress inducers and of the modulators of antioxidant status. This concept was also applied to the study of the effects of well-known antioxidants, such as vitamin E, quercetin, and lipoic acid. Our results confirm the zebrafish model as an in vivo useful tool to test the protective effects of antioxidant compounds.

【 授权许可】

Unknown