Revealing Clonal Responses of Tumor-Reactive T-Cells Through T Cell Receptor Repertoire Analysis

Frontiers in Immunology

Hiroyasu Aoki¹  Shigeyuki Shichino²  Kouji Matsushima²  Satoshi Ueha² 

[1] Department of Hygiene, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan;Division of Molecular Regulation of Inflammatory and Immune Diseases, Research Institute for Biomedical Sciences, Tokyo University of Science, Chiba, Japan;

关键词: CD8+ T cell;    T-cell receptor repertoire;    single-cell TCR-seq;    immune check inhibitors;    cancer-immunity cycle;    inter-organ clone tracking;   

DOI  :  10.3389/fimmu.2022.807696

来源: DOAJ

【 摘 要 】

CD8+ T cells are the key effector cells that contribute to the antitumor immune response. They comprise various T-cell clones with diverse antigen-specific T-cell receptors (TCRs). Thus, elucidating the overall antitumor responses of diverse T-cell clones is an emerging challenge in tumor immunology. With the recent advancement in next-generation DNA sequencers, comprehensive analysis of the collection of TCR genes (TCR repertoire analysis) is feasible and has been used to investigate the clonal responses of antitumor T cells. However, the immunopathological significance of TCR repertoire indices is still undefined. In this review, we introduce two approaches that facilitate an immunological interpretation of the TCR repertoire data: inter-organ clone tracking analysis and single-cell TCR sequencing. These approaches for TCR repertoire analysis will provide a more accurate understanding of the response of tumor-specific T cells in the tumor microenvironment.

【 授权许可】

Unknown