Isolation of Fungi from a Textile Industry Effluent and the Screening of Their Potential to Degrade Industrial Dyes

Journal of Fungi

Dalia Castillo-Hernández¹  Martha Bibbins-Martínez¹  Edy Surian-Cruz¹  Soley Nava-Galicia¹  Cristhian Pérez-Parada¹  Juvenal Juárez-Hernández¹  Jaime Alioscha Cuervo-Parra²  Gerardo Díaz-Godínez³  Carmen Sánchez³ 

[1] Centro de Investigación en Biotecnología Aplicada, Instituto Politécnico Nacional, Tepetitla de Lardizabal 90700, Tlaxcala, Mexico;Escuela Superior de Apan, Universidad Autónoma del Estado de Hidalgo, Carretera Apan-Calpulalpan, Chimalpa Tlalayote, Apan 43900, Hidalgo, Mexico;Laboratorio de Biotecnología, Centro de Investigación en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala 90120, Tlaxcala, Mexico;

关键词: Emmia latemarginata;    biodegradation;    decolorization;    industrial dyes;    textile effluent;    ligninolytic enzymes;   

DOI  :  10.3390/jof7100805

来源: DOAJ

【 摘 要 】

Six fungal strains were isolated from the textile industry effluent in which they naturally occur. Subsequently, the fungal strains were identified and characterized in order to establish their potential decolorizing effect on textile industry effluents. The strains of interest were selected based on their capacity to decolorize azo, indigo, and anthraquinone dyes. Three of the strains were identified as Emmia latemarginata (MAP03, MAP04, and MAP05) and the other three as Mucor circinelloides (MAP01, MAP02, and MAP06), while the efficiency of their decolorization of the dyes was determined on agar plate and in liquid fermentation. All the strains co-metabolized the dyes of interest, generating different levels of dye decolorization. Plate screening for lignin-degrading enzymes showed that the MAP03, MAP04, and MAP05 strains were positive for laccase and the MAP01, MAP02, and MAP06 strains for tyrosinase, while all strains were positive for peroxidase. Based on its decolorization capacity, the Emmia latemarginata (MAP03) strain was selected for the further characterization of its growth kinetics and ligninolytic enzyme production in submerged fermentation under both enzyme induction conditions, involving the addition of Acetyl yellow G (AYG) dye or wheat straw extract, and no-induction condition. The induction conditions promoted a clear inductive effect in all of the ligninolytic enzymes analyzed. The highest level of induced enzyme production was observed with the AYG dye fermentation, corresponding to versatile peroxidase (VP), manganese peroxidase (MnP), and lignin peroxidase (LiP). The present study can be considered the first analysis of the ligninolytic enzyme system of Emmia latemarginata in submerged fermentation under different conditions. Depending on the results of further research, the fungal strains analyzed in the present research may be candidates for further biotechnological research on the decontamination of industrial effluents.

【 授权许可】

Unknown