Differential Accumulation of Innate- and Adaptive-Immune-Response-Derived Transcripts during Antagonism between Papaya Ringspot Virus and Papaya Mosaic Virus

Viruses

Gabriela Chávez-Calvillo¹  Laura Silva-Rosales¹  Julio Vega-Arreguín²  Pablo Vargas-Mejía²  Enrique Ibarra-Laclette³ 

[1] Departamento de Ingeniería Genética, Centro de Investigaciones y de Estudios Avanzados, Unidad Irapuato Libramiento Norte Carretera Irapuato-León, Irapuato, Gto. 36824, Mexico;Laboratorio de Ciencias AgroGenómicas, Escuela Nacional de Estudios Superiores, Universidad Nacional Autónoma de México, León, Gto. 37684, Mexico;Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C. Carretera Antigua a Coatepec 351, Xalapa, Ver. 91070, Mexico;

关键词: antagonism;    cross protection;    prsv;    papmv;    host resistance;    plant–virus interaction;    potyvirus;    superinfection exclusion;    papaya genomics;   

DOI  :  10.3390/v12020230

来源: DOAJ

【 摘 要 】

Papaya ringspot virus (PRSV), a common potyvirus infecting papaya plants worldwide, can lead to either antagonism or synergism in mixed infections with Papaya mosaic virus (PapMV), a potexvirus. These two unrelated viruses produce antagonism or synergism depending on their order of infection in the plant. When PRSV is inoculated first or at the same time as PapMV, the viral interaction is synergistic. However, an antagonistic response is observed when PapMV is inoculated before PRSV. In the antagonistic condition, PRSV is deterred from the plant and its drastic effects are overcome. Here, we examine differences in gene expression by high-throughput RNA sequencing, focused on immune system pathways. We present the transcriptomic expression of single and mixed inoculations of PRSV and PapMV leading to synergism and antagonism. Upregulation of dominant and hormone-mediated resistance transcripts suggests that the innate immune system participates in synergism. In antagonism, in addition to innate immunity, upregulation of RNA interference-mediated resistance transcripts suggests that adaptive immunity is involved.

【 授权许可】

Unknown