Flow Dynamics and HSPC Homing in Bone Marrow Microvessels

Cell Reports

Anjali P. Kusumbe¹  Ralf. H. Adams¹  M. Gabriele Bixel¹  Kishor K. Sivaraj¹  Saravana K. Ramasamy¹  Stefan Butz²  Dietmar Vestweber² 

[1] Department of Tissue Morphogenesis, Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany;Department of Vascular Cell Biology, Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany;

关键词: intravital imaging;    deep tissue imaging;    bone marrow;    microvasculature;    blood flow velocities;    wall shear stress;    hematopoietic parameters;    hematopoietic stem cell;    stem cell homing;   

DOI  :  10.1016/j.celrep.2017.01.042

来源: DOAJ

【 摘 要 】

Measurements of flow velocities at the level of individual arterial vessels and sinusoidal capillaries are crucial for understanding the dynamics of hematopoietic stem and progenitor cell homing in the bone marrow vasculature. We have developed two complementary intravital two-photon imaging approaches to determine blood flow dynamics and velocities in multiple vessel segments by capturing the motion of red blood cells. High-resolution spatiotemporal measurements through a cranial window to determine short-time dynamics of flowing blood cells and repetitive centerline scans were used to obtain a detailed flow-profile map with hemodynamic parameters. In addition, we observed the homing of individual hematopoietic stem and progenitor cells and obtained detailed information on their homing behavior. With our imaging setup, we determined flow patterns at cellular resolution, blood flow velocities and wall shear stress in small arterial vessels and highly branched sinusoidal capillaries, and the cellular dynamics of hematopoietic stem and progenitor cell homing.

【 授权许可】

Unknown