期刊论文详细信息
Journal of Fungi
Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock
Ayixón Sánchez-Reyes1  Nildadel C. Sánchez2  Yordanis Pérez-Llano3  JorgeLuis Folch-Mallol3  Maríadel Rocío Rodríguez-Hernández3  Maríadel Rayo Sánchez-Carbente3  EyaCaridad Rodríguez-Pupo3  RamónAlberto Batista-García4  JoséRaunel Tinoco-Valencia5  Martha Calahorra6  NormaSilvia Sánchez6  Antonio Peña6  Jesús Aguirre6  Francisco Padilla-Garfias6  Olivia Sánchez6 
[1] Catedras Conacyt-Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Campus Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca C.P. 62210, Morelos, Mexico;Centro de Ciencias Genómicas, UNAM, Campus Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca C.P. 62210, Morelos, Mexico;Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos (UAEM), Av. Universidad 1001, Col. Chamilpa, Cuernavaca C.P. 62209, Morelos, Mexico;Centro de Investigación en Dinámica Celular, IICBA, UAEM, Av. Universidad 1001, Col. Chamilpa, Cuernavaca C.P. 62209, Morelos, Mexico;Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Campus Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca C.P. 62210, Morelos, Mexico;Instituto de Fisiología Celular, UNAM, Cto. Exterior s/n, Cd. Universitaria, Coyoacán, Ciudad de México C.P. 04510, Federal District, Mexico;
关键词: halophile;    osmolyte;    osmotic shock;    HOG;    Aspergillus;    extremophile;   
DOI  :  10.3390/jof7060414
来源: DOAJ
【 摘 要 】

Aspergillus sydowii is a moderate halophile fungus extensively studied for its biotechnological potential and halophile responses, which has also been reported as a coral reef pathogen. In a recent publication, the transcriptomic analysis of this fungus, when growing on wheat straw, showed that genes related to cell wall modification and cation transporters were upregulated under hypersaline conditions but not under 0.5 M NaCl, the optimal salinity for growth in this strain. This led us to study osmolyte accumulation as a mechanism to withstand moderate salinity. In this work, we show that A. sydowii accumulates trehalose, arabitol, mannitol, and glycerol with different temporal dynamics, which depend on whether the fungus is exposed to hypo- or hyperosmotic stress. The transcripts coding for enzymes responsible for polyalcohol synthesis were regulated in a stress-dependent manner. Interestingly, A. sydowii contains three homologs (Hog1, Hog2 and MpkC) of the Hog1 MAPK, the master regulator of hyperosmotic stress response in S. cerevisiae and other fungi. We show a differential regulation of these MAPKs under different salinity conditions, including sustained basal Hog1/Hog2 phosphorylation levels in the absence of NaCl or in the presence of 2.0 M NaCl, in contrast to what is observed in S. cerevisiae. These findings indicate that halophilic fungi such as A. sydowii utilize different osmoadaptation mechanisms to hypersaline conditions.

【 授权许可】

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