Analytical Characterization of an Inulin-Type Fructooligosaccharide from Root-Tubers of Asphodelusramosus L

Pharmaceuticals

Francesco Saccoliti¹  Chiara Toniolo²  Daniela De Vita²  Manuela Grimaldi³  AnnaMaria D’Ursi³  Roberto Di Santo⁴  Ivano Pindinello⁴  ValentinaNoemi Madia⁴  Valeria Tudino⁴  Luigi Scipione⁴  Davide Ialongo⁴  Alessandro De Leo⁴  Roberta Costi⁴  Antonella Messore⁴  Pietro Ceccobelli⁵ 

[1] D3 PharmaChemistry, Italian Institute of Technology, Via Morego 30, 16163 Genova, Italy;Department of Environmental Biology, “Sapienza” University of Rome, p.le Aldo Moro 5, 00185 Rome, Italy;Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, Fisciano, 84084 Salerno, Italy;Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Chimica e Tecnologie del Farmaco, “Sapienza” Università di Roma, p.le Aldo Moro 5, 00185 Rome, Italy;Local Health Authority (ASL)/rm202, 00157 Rome, Italy;

关键词: Asphodelus ramosus;    root-tubers;    inulin;    fructans;    fructooligosaccharide;    alkaline extraction;   

DOI  :  10.3390/ph14030278

来源: DOAJ

【 摘 要 】

Plant-based systems continue to play a pivotal role in healthcare, and their use has been extensively documented. Asphodelus L. is a genus comprising various herbaceous species, known by the trivial name Asphodelus. These plants have been known since antiquity for both food and therapeutic uses, especially for treating several diseases associated with inflammatory and infectious skin disorders. Phytochemical studies revealed the presence of different constituents, mainly anthraquinones, triterpenoids, phenolic acids, and flavonoids. Although extensive literature has been published on these constituents, a paucity of information has been reported regarding the carbohydrate composition, such as fructans and fructan-like derivatives. The extraction of water-soluble neutral polysaccharides is commonly performed using water extraction, at times assisted by microwaves and ultrasounds. Herein, we reported the investigation of the alkaline extraction of root-tubers of Asphodelus ramosus L., analyzing the water-soluble polysaccharides obtained by precipitation from the alkaline extract and its subsequent purification by chromatography. A polysaccharide was isolated by alkaline extraction; the HPTLC study to determine its composition showed fructose as the main monosaccharide. FT-IR analysis showed the presence of an inulin-type structure, and NMR analyses allowed us to conclude that A. ramosus roots contain polysaccharide with an inulin-type fructooligosaccharide with a degree of polymerization of 7–8.

【 授权许可】

Unknown