Evaluation of the mutagenicity and antimutagenicity of Ziziphus joazeiro Mart. bark in the micronucleus assay

Genetics and Molecular Biology

Nelma de Mello Silva Oliveira¹  João Evangelista Fiorini¹  Luiz Silva Souza¹  Thaísla Andrielle da Silva¹  Juliana Yoshida Públio¹  Marcelo Fabiano Gomes Boriollo¹  Carlos Tadeu dos Santos Dias²  Marielly Reis Resende² 

[1] Universidade José do Rosário Vellano;Universidade de São Paulo;

关键词: antimutagenicity;    bone marrow;    doxorubicin;    micronucleus assay;    mutagenicity;    Zizyphus joazeiro Mart. (raspa-de-Juá);   

DOI  :  10.1590/S1415-47572014000300016

来源: DOAJ

【 摘 要 】

The aim of this study was to evaluate the mutagenicity (clastogenicity/aneugenicity) of a glycolic extract of Ziziphus joazeiro bark (GEZJ) by the micronucleus assay in mice bone marrow. Antimutagenic activity was also assessed using treatments associated with GEZJ and doxorubicin (DXR). Mice were evaluated 24-48 h after exposure to positive (N-nitroso-N-ethylurea, NEU - 50 mg.kg-1 and DXR - 5 mg.kg-1) and negative (150 mM NaCl) controls, as well as treatment with GEZJ (0.5-2 g.kg-1), GEZJ (2 g.kg-1) + NEU and GEZJ (2 g.kg-1) + DXR. There were no significant differences in the frequencies of micronucleated polychromatic erythrocytes in mice treated with GEJZ and GEJZ + DXR compared to the negative controls, indicating that GEZJ was not mutagenic. Analysis of the polychromatic:normochromatic erythrocyte ratio revealed significant differences in the responses to doses of 0.5 g.kg-1 and 1-2 g.kg-1 and the positive control (NEU). These results indicated no systemic toxicity and moderate toxicity at lower and higher doses of GEZJ. The lack of mutagenicity and systemic toxicity in the antimutagenic assays, especially for treatment with GEZJ + DXR, suggested that phytochemical compounds in Z. joazeiro bark attenuated DXR-induced mutagenicity and the moderate systemic toxicity of a high dose of Z. joazeiro bark (2 g.kg-1). Further studies on the genotoxicity of Z. joazeiro extracts are necessary to establish the possible health risk in humans and to determine the potential as a chemopreventive agent for therapeutic use.

【 授权许可】

Unknown