Ascospore discharge, germination and culture of fungal partners of tropical lichens, including the use of a novel culture technique

IMA Fungus

关键词: Ascomycota;    colony development;    mycobiont;    seasonality;    Thailand;   

DOI  :  

来源: DOAJ

【 摘 要 】

A total of 292 lichen samples, representing over 200 species and at least 65 genera and 26 families, were collected, mainly in Thailand; 170 of the specimens discharged ascospores in the laboratory. Generally,crustose lichens exhibited the highest discharge rates and percentage germination. In contrast, foliose lichen samples, although having a high discharge rate, had a lower percentage germination than crustose species tested. A correlation with season was indicated for a number of species. Continued development of germinated ascospores into recognizable colonies in pure culture was followed for a selection of species. The most successful medium tried was 2 % Malt-Yeast extract agar (MYA), and under static conditions using a liquid culture medium, a sponge proved to be the best of several physical carriers tested; this novel method has considerable potential for experimental work with lichen mycobionts.

【 授权许可】

Unknown