Expression of Transforming Growth Factor Beta Isoforms in Canine Endometrium with Cystic Endometrial Hyperplasia–Pyometra Complex

Animals

Marek Skrzypski¹  Jędrzej M. Jaśkowski²  Tomasz Nowak³  Magdalena Kubiak⁴  Magdalena Woźna-Wysocka⁵  Marta Rybska⁶  Anna Łukomska⁶  Barbara Wąsowska⁶  Beata Błaszak⁷ 

[1] Department of Animal Physiology, Biochemistry and Biostructure, Poznan University of Life Sciences, Wołyńska 35, 60-637 Poznan, Poland;Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University, Gagarina 7, 87-100 Torun, Poland;Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Wołyńska 35, 60-637 Poznan, Poland;Department of Internal Diseases and Diagnostics, Poznan University of Life Sciences, Wołyńska 35, 60-637 Poznan, Poland;Department of Medical Biotechnology, Institute of Bioorganic Chemistry Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland;Department of Preclinical Sciences and Infectious Diseases, Poznan University of Life Science, Wołyńska 35, 60-637 Poznan, Poland;Department of Tumour Pathology, Greater Poland Cancer Centre, Garbary 15, 61-866 Poznan, Poland;

关键词: pyometra;    cystic endometrial hyperplasia;    canine;    endometrium;    uterus;    transforming growth factor beta;   

DOI  :  10.3390/ani11061844

来源: DOAJ

【 摘 要 】

Cystic endometrial hyperplasia (CEH) and pyometra are the most frequently diagnosed uterine diseases affecting bitches of different ages. Transforming growth factor beta (TGF-β) has been classified in females as a potential regulator of many endometrial changes during the estrous cycle or may be involved in pathological disorders. The aim of this study was to determine the expression of TGF-β1, -β2 and -β3 in the endometrium of bitches suffering from CEH or a CEH–pyometra complex compared to clinically healthy females (control group; CG). A significantly increased level of TGF-β1 mRNA expression was observed in the endometrium with CEH–pyometra compared to CEH and CG. Protein production of TGF-β1 was identified only in the endometrium of bitches with CEH–pyometra. An increase in TGF-β3 mRNA expression was observed in all the studied groups compared to CG. The expression of TGF-β2 mRNA was significantly higher in CEH and lower in CEH–pyometra uteri. The results indicate the presence of TGF-β cytokines in canine endometrial tissues affected by proliferative and degenerative changes. However, among all TGF-β isoforms, TGF-β1 could potentially be a key factor involved in the regulation of the endometrium in bitches with CEH–pyometra complex.

【 授权许可】

Unknown