Artificial Heme Enzymes for the Construction of Gold-Based Biomaterials

International Journal of Molecular Sciences

Angela Lombardi¹  Vincenzo Pavone¹  Emmanuel Ruggiero¹  Marco Chino¹  Anna Malafronte¹  Gerardo Zambrano¹  Ornella Maglio¹  Flavia Nastri¹ 

[1] Department of Chemical Sciences, University of Napoli “Federico II” Via Cintia, 80126 Napoli, Italy;

关键词: gold nanoparticles;    artificial enzymes;    immobilization strategies;    catalytic activity;    peroxidases;    functional biomaterials;   

DOI  :  10.3390/ijms19102896

来源: DOAJ

【 摘 要 】

Many efforts are continuously devoted to the construction of hybrid biomaterials for specific applications, by immobilizing enzymes on different types of surfaces and/or nanomaterials. In addition, advances in computational, molecular and structural biology have led to a variety of strategies for designing and engineering artificial enzymes with defined catalytic properties. Here, we report the conjugation of an artificial heme enzyme (MIMO) with lipoic acid (LA) as a building block for the development of gold-based biomaterials. We show that the artificial MIMO@LA can be successfully conjugated to gold nanoparticles or immobilized onto gold electrode surfaces, displaying quasi-reversible redox properties and peroxidase activity. The results of this work open interesting perspectives toward the development of new totally-synthetic catalytic biomaterials for application in biotechnology and biomedicine, expanding the range of the biomolecular component aside from traditional native enzymes.

【 授权许可】

Unknown