Holmium-Containing Bioactive Glasses Dispersed in Poloxamer 407 Hydrogel as a Theragenerative Composite for Bone Cancer Treatment

Materials

AnaC. S. de Souza¹  Roger Borges¹  Telma Zambanini¹  DanieleR. de Araujo¹  Juliana Marchi¹  GiselleZ. Justo²  Joel Machado³ 

[1] Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André 09210-580, SP, Brazil;Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo 04044-020, SP, Brazil;Departamento de Ciências Biológicas, Universidade Federal de São Paulo, Diadema 04039-032, SP, Brazil;

关键词: bone cancer;    brachytherapy;    bioactive glass;    holmium;    hydrogel;    theragenerative biomaterial;   

DOI  :  10.3390/ma14061459

来源: DOAJ

【 摘 要 】

Holmium-containing bioactive glasses can be applied in bone cancer treatment because the holmium content can be neutron activated, having suitable properties for brachytherapy applications, while the bioactive glass matrix can regenerate the bone alterations induced by the tumor. To facilitate the application of these glasses in clinical practice, we proposed a composite based on Poloxamer 407 thermoresponsive hydrogel, with suitable properties for applications as injectable systems. Therefore, in this work, we evaluated the influence of holmium-containing glass particles on the properties of Poloxamer 407 hydrogel (20 w/w.%), including self-assembly ability and biological properties. 58S bioactive glasses (58SiO₂-33CaO-9P₂O₅) containing different Ho₂O₃ amounts (1.25, 2.5, 3.75, and 5 wt.%) were incorporated into the hydrogel. The formulations were characterized by scanning electron microscopy, differential scanning calorimetry, rheological tests, and [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] MTT cell viability against pre-osteoblastic and osteosarcoma cells. The results evidenced that neither the glass particles dispersed in the hydrogel nor the holmium content in the glasses significantly influenced the hydrogel self-assembly ability (T_mic ~13.8 °C and T_gel ~20 °C). Although, the glass particles considerably diminished the hydrogel viscosity in one order of magnitude at body temperature (37 °C). The cytotoxicity results evidenced that the formulations selectively favored pre-osteoblastic cell proliferation and osteosarcoma cell death. In conclusion, the formulation containing glass with the highest fraction of holmium content (5 wt.%) had the best biological results outcomes aiming its application as theragenerative materials for bone cancer treatment.

【 授权许可】

Unknown