Prolonged Viability of Senecavirus A in Exposed House Flies (Musca domestica)

Viruses

Justin Talley¹  Fernando Vicosa Bauermann²  Justin Heath Turner²  Mayara Fernanda Maggioli²  Cristina Mendes Peter²  Willian Pinto Paim²  Robert Miknis³ 

[1] Department of Entomology & Plant Pathology, Ferguson College of Agriculture, Oklahoma State University (OSU), Stillwater, OK 74078, USA;Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University (OSU), Stillwater, OK 74078, USA;United States Department of Agriculture Animal and Plant Inspection Service Veterinary Service Strategy and Policy Office of Interagency Coordination, Fort Collins, CO 80526, USA;

关键词: insect;    house flies;    mechanical vector;    Picornaviridae;    Poxviridae;    virus tenacity;   

DOI  :  10.3390/v14010127

来源: DOAJ

【 摘 要 】

House flies (Musca domestica) are often present in swine farms worldwide. These flies utilize animal secretions and waste as a food source. House flies may harbor and transport microbes and pathogens acting as mechanical vectors for diseases. Senecavirus A (SVA) infection in pigs occurs via oronasal route, and animals shed high virus titers to the environment. Additionally, SVA possesses increased environmental resistance. Due to these reasons, we investigated the tenacity of SVA in house flies. Five groups of flies, each composed of ten females and ten males, were exposed to SVA, titer of 10^9.3 tissue culture infectious dose (TCID₅₀/mL). Groups of male and female flies were collected at 0, 6, 12, 24, and 48 h post-exposure. For comparison purposes, groups of flies were exposed to Swinepox virus (SwPV). Infectious SVA was identified in all tested groups. Successful isolation of SVA demonstrated the titers varied between 10^6.8 and 10^2.8 TCID₅₀/mL in female groups and varied from 10^5.85 to 10^3.8 TCID₅₀/mL in male groups. In contrast, infectious SwPV was only detected in the female group at 6 h. The significant SVA infectious titer for prolonged periods of time, up to 48 h, indicates a potential role of flies in SVA transmission.

【 授权许可】

Unknown