Natterin-like depletion by CRISPR/Cas9 impairs zebrafish (Danio rerio) embryonic development

BMC Genomics

Milena Marcolino Souza¹  Maria Alice Pimentel Falcao¹  Monica Lopes-Ferreira¹  Adolfo Luis Almeida Maleski¹  Carla Lima¹  Ana Carolina Seni-Silva¹  Geonildo Rodrigo Disner¹ 

[1] Immunoregulation Unit of the Laboratory of Applied Toxinology (CeTICs/FAPESP), Butantan Institute;

关键词: Natterin proteins family;    Danio rerio;    Embryogenesis;    CRISPR/Cas9;    Phenotype-based screening;    Physiological functions;   

DOI  :  10.1186/s12864-022-08369-z

来源: DOAJ

【 摘 要 】

Highlights The Natterin family was discovered in the venom of the fish Thalassophryne nattereri. The zebrafish genome encodes eleven natterin-like genes. Natterin-like might be a novel fish-specific defense molecule. Natterin-like proteins are thought to be pore-forming molecules. Reverse genetic study and phenotypic characterization suggests natterin-like genes may have roles in zebrafish development.

【 授权许可】

Unknown