Identification of the Potential Key Long Non-coding RNAs in Aged Mice With Postoperative Cognitive Dysfunction

Frontiers in Aging Neuroscience

Zheng Zhang¹  Rui Gao¹  Qiao Wang¹  Chan Chen¹  Lu Gan¹  Ming Li¹  Hai Yu¹  Jin Liu¹  Tao Zhu¹  Weiyi Zhang¹  Hai Chen¹  Shu Zhang²  Adam Behensky³  Mathis Leblanc³  Xiaobo Mao³ 

[1] Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, China;Department of Emergency Medicine, West China Hospital, Sichuan University, Chengdu, China;Institute of Cell Engineering, Department of Neurology, School of Medicine, Johns Hopkins University, Baltimore, MD, United States;

关键词: long non-coding RNA;    microarray;    postoperative cognitive dysfunction;    aging;    inflammation;    apoptosis;   

DOI  :  10.3389/fnagi.2019.00181

来源: DOAJ

【 摘 要 】

Postoperative cognitive dysfunction (POCD) is a significant complication of surgery, particularly in elderly patients. Emerging researches showed that long non-coding RNA (lncRNA) may play a vital role in the pathogenesis of POCD. Here we aimed to identify potential key lncRNAs involved in the development of POCD. LncRNA and mRNA expression profiles in hippocampal tissues from POCD and control mice were analyzed by microarray assay. Gene ontology (GO) and KEGG pathway enrichment analyses were conducted to probe the functions of dysregulated genes. Then, important factors of the mainly affected biological processes were measured in the hippocampus. Correlated coding–non-coding co-expression (CNC) networks were constructed. Finally, the potential key pairs of lncRNA and target mRNA implicated in POCD were probed. Our data showed that 868 differentially expressed lncRNAs and 690 differentially expressed mRNAs were identified in total. GO and KEGG analyses indicated that the differentially expressed genes were mainly associated with inflammatory and apoptotic signaling pathways. Surgery-induced inflammatory cytokines and apoptosis were significantly increased in hippocampal tissues of aged mice. In CNC network analysis, we found that LncRNA uc009qbj.1 was positively correlated with apoptosis-associated gene Vrk2 level. LncRNA ENSMUST00000174338 correlated positively with expression of the inflammation and apoptosis-associated gene Smad7. LncRNA NONMMUT00000123687 mediated gene expression by binding the inflammation-regulated transcription factor Meis2. Our results suggested that these potential key lncRNAs and mRNAs may play a crucial role in the development of POCD through mediating neuronal inflammation or apoptosis.

【 授权许可】

Unknown