A nanocomplex of C60 fullerene with cisplatin: design, characterization and toxicity

Beilstein Journal of Nanotechnology

Maxim Evstigneev¹  Viktor Kostjukov²  Volodymyr Korolovych³  Kateryna Afanasieva⁴  Andriy Sivolob⁴  Larysa Skivka⁴  Kateryna Bogutska⁴  Svitlana Prylutska⁴  Yuriy Prylutskyy⁴  Svitlana Politenkova⁴  Uwe Ritter⁵ 

[1] Belgorod State University, Pobedy Str. 85, 308015 Belgorod, Russia;Department of Physics, Sevastopol State University, Sevastopol 299053, Crimea;School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, USA;Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01601 Kyiv, Ukraine;Technical University of Ilmenau, Institute of Chemistry and Biotechnology, Weimarer Str., 25, 98693 Ilmenau, Germany;

关键词: atomic force microscopy;    C60 fullerene;    cisplatin;    comet assay;    computer simulation;    dynamic light scattering;    flow cytometry;    human lymphocytes;    toxicity in vitro;   

DOI  :  10.3762/bjnano.8.149

来源: DOAJ

【 摘 要 】

The self-organization of C60 fullerene and cisplatin in aqueous solution was investigated using the computer simulation, dynamic light scattering and atomic force microscopy techniques. The results evidence the complexation between the two compounds. The genotoxicity of С60 fullerene, Cis and their complex was evaluated in vitro with the comet assay using human resting lymphocytes and lymphocytes after blast transformation. The cytotoxicity of the mentioned compounds was estimated by Annexin V/PI double staining followed by flow cytometry. The results clearly demonstrate that water-soluble C60 fullerene nanoparticles (0.1 mg/mL) do not induce DNA strand breaks in normal and transformed cells. C60 fullerene in the mixture with Cis does not influence genotoxic Cis activity in vitro, affects the cell-death mode in treated resting human lymphocytes and reduces the fraction of necrotic cells.

【 授权许可】

Unknown