Molecular identification of four medicinal plants using DNA barcoding approach from Chittagong, Bangladesh

Journal of Advanced Biotechnology and Experimental Therapeutics

BaishakhiBiswas¹  SabrinaAmin¹  SrabastiGhosh¹  Md. Arifuzzaman¹  Md.Abul Kalam Azad¹  AMAM ZonaedSiddiki² 

[1] Department of Biochemistry and Biotechnology, Faculty of Basic Medical and Pharmaceutical Sciences, University of Science and Technology Chittagong USTC, Foys Lake, Khulshi4202, Chittagong, Bangladesh. ;Genomics Research Group, Chittagong Veterinary and Animal Science University, Khulshi -4225, Chittagong, Bangladesh.;

关键词: dna barcode;    medicinal plant;    matk gene;    species identification;    phylogeny;   

DOI  :  10.5455/jabet.2020.d134

来源: DOAJ

【 摘 要 】

Accurate identification of important plants is essential for their safety, efficacy and herbal remedies. A total of four medicinal plants were attempted to be identified using DNA barcoding approach during this study. Genomic DNA was extracted from plant samples followed by their amplification by conventional PCR approach. Short sequence diversity of standardized specific coding gene regions of matK gene of plastid genome was used to compare and differentiate the plant species. Subsequently, all the samples were purified and sequenced successfully. A phylogenetic tree was constructed to assess their cross-species relationship. All the samples showed high similarity rate with their homologues after blast them in NCBI database. Phylogenetic study showed distinguish relationship with each other. All the result indicates that DNA barcoding approach could be successfully used for reliable identification of medicinal plants and matK gene is a good candidate for this approach. [ J Adv Biotechnol Exp Ther 2020; 3(3.000): 268-272]

【 授权许可】

Unknown