A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods

Journal of Food and Drug Analysis

Nai-Dong Chen¹  Jing-Wen Hao¹  Li-Tao Bai¹  Xiao-Yuan Xu¹  Jun Li²  Tao You² 

[1] College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu'an City, China;College of Pharmacy, Anhui Medical University, Hefei, China;

关键词: Dendrobium huoshanense;    Dendrobium moniliforme;    Dendrobium officinale;    gas chromatography–mass spectrometry;    principal component analysis;   

DOI  :  10.1016/j.jfda.2016.05.006

来源: DOAJ

【 摘 要 】

Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences. In this paper, a headspace gas chromatography–mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.) Sw. In total, 63 volatile compounds were separated, with 53 being identified from the three Dendrobium spp. samples. Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions. The similarity evaluation disclosed that the accumulation of volatile compounds in Dendrobium samples might be affected by their provenance. Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples. Gas chromatography–mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.

【 授权许可】

Unknown