In Vitro and In Vivo Antitumor Efficacy of Hizikia fusiforme Celluclast Extract against Bladder Cancer

Nutrients

YungHyun Choi¹  Sung-Soo Park²  Soontag Jung³  SeYeon Won³  Sung-Kwon Moon³  Jun-Hui Song³  Changsun Choi³  Byungdoo Hwang³  Wun-Jae Kim⁴ 

[1] Department of Biochemistry, College of Oriental Medicine, Dongeui University, Busan 47340, Korea;Department of Food Science and Nutrition, Jeju National University, Jeju 63243, Korea;Department of Food and Nutrition, Chung-Ang University, Anseong 17546, Korea;Department of Urology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea;

关键词: Hizikia fusiforme;    bladder cancer;    EJ;    cell cycle;    JNK;    migration;   

DOI  :  10.3390/nu12072159

来源: DOAJ

【 摘 要 】

Various physiological benefits have been linked to Hizikia fusiforme (HF), an edible brown seaweed. Here, fucose-containing sulfated polysaccharides were extracted from celluclast-processed HF (SPHF) and their antitumor efficacy against bladder cancer was evaluated in vitro and in vivo. SPHF possesses high sulfated polysaccharide and fucose contents and free radical scavenging activities compared to those of celluclast-processed HF extracts (CHF). SPHF inhibited bladder cancer EJ cell proliferation via G1-phase cell cycle arrest. This was due to the induction of p21WAF1 expression associated with the downregulation of CDKs and cyclins. Moreover, JNK phosphorylation was identified as an SPHF-mediated signaling molecule. SPHF treatment also hindered the migration and invasion of EJ cells by inhibiting MMP-9 expression, which was attributed to the repression of transcriptional binding to NF-κB, AP-1, and Sp-1 in the MMP-9 promoter region. In an animal study, SPHF treatment suppressed EJ tumor growth in xenograft mice similarly to cisplatin. Furthermore, no toxicity signs were found after weight loss assessment, biochemical tests, and organ tissue immunostaining during oral administration of 20–200 mg/kg SPHF for 20 days. Therefore, our study demonstrates the antitumor efficacy of SPHF in vitro and in vivo, thus highlighting its potential for bladder cancer treatment development.

【 授权许可】

Unknown