Molecular features of hepatitis E virus from farmed rabbits in Shandong province, China

World Rabbit Science

Yufa Zhou¹  Hongna Zhang²  Jingbo Liu³ 

[1] Center for Disease Control, Daiyue Bureau of Animal Husbandry;Hebei University of Economics and Business;Institute of Microbiology Research, Baolai-Leelai Bio-Tech. Ltd.;

关键词: HEV;    farmed rabbit;    RT-nPCR;    phylogenetic analysis;    nucleotide identity;   

DOI  :  10.4995/wrs.2018.10225

来源: DOAJ

【 摘 要 】

This study was undertaken to investigate the genetic variability of hepatitis E virus (HEV) from farmed rabbits in Shandong province, China. A total of 50 fresh faecal samples from 5 rabbit farms were collected and subjected to reverse transcription and nested polymerase chain reaction (RT-nPCR) for a fragment sequence of HEV capsid gene. The results demonstrated that HEV RNA was observed in 6 faecal samples (6/50, 12.0%). In addition, the result of phylogenetic analysis showed that the 6 HEV isolates were classified into HEV-3 genotype with other rabbit HEV isolates from other countries, and shared 85.2-87.2%, 81.5-83.1%, and 77.0-78.6% nucleotide similarities with rabbit HEV isolates from Korea, the United States and France, respectively. To sum up, the HEV isolated in this study from farmed rabbits belongs to the HEV-3 genotype, and the zoonotic ability and pathogenesis of the rabbit HEV merit further study due to the fact that HEV-3 genotype has the potential to trigger zoonotic infections.

【 授权许可】

Unknown