Unravelling the Molecular Identification and Antifungal Susceptibility Profiles of Aspergillus spp. Isolated from Chronic Pulmonary Aspergillosis Patients in Jakarta, Indonesia: The Emergence of Cryptic Species

Journal of Fungi

Anom Bowolaksono¹  Wellyzar Sjamsuridzal¹  Retno Wahyuningsih²  Ayu Eka Fatril²  Findra Setianingrum²  Heri Wibowo²  Robiatul Adawiyah²  Mulyati Tugiran²  Ridhawati Syam²  Anna Rozaliyani²  Asriyani Abdullah³  David W. Denning⁴  Chris Kosmidis⁴ 

[1] Department of Biology, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia, Depok 16424, Indonesia;Department of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia;Magister Program of Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia;Manchester Fungal Infection Group, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M23 9LT, UK;

关键词: Aspergillus;    cryptic;    antifungal susceptibility;    tuberculosis;    chronic pulmonary aspergillosis;   

DOI  :  10.3390/jof8040411

来源: DOAJ

【 摘 要 】

Cryptic species of Aspergillus have rapidly increased in the last few decades. Chronic pulmonary aspergillosis (CPA) is a debilitating fungal infection frequently affecting patients with previous TB. The identification and antifungal susceptibility profiles of different species of Aspergillus are important to support the management of CPA. The aim of this study was to describe the molecular and susceptibility profiles of Aspergillus isolated from CPA patients. The species identity of isolates was determined by combined DNA analyses of internal transcribed space (ITS), partial β-tubulin genes, and part of the calmodulin gene. We revealed a high (27%) prevalence of cryptic species among previous tuberculosis patients with persistent symptoms. Twenty-nine (49%) patients met the criteria for diagnosis of CPA with 24% containing Aspergillus cryptic species. This is the first report of five cryptic Aspergillus species from clinical isolates in Indonesia: A. aculea tus, A. neoniger, A. brunneoviolacues, A. welwitschiae, and A. tubingensis. Significantly, there was decreased sensitivity against itraconazole in the CPA group (66% susceptible to itraconazole) compared to the non-CPA group (90% susceptible to itraconazole) (p = 0.003). The species-level characterisation of Aspergillus and its antifungal susceptibility tests demands greater attention to better the management of CPA patients.

【 授权许可】

Unknown