Characteristics and Genomic Diversity of Measles Virus From Measles Cases With Known Vaccination Status in Shanghai, China

Frontiers in Medicine

Yang Li¹  Xiaodong Sun²  Zhi Li²  Zhuoying Huang²  Xinyi Cui³  Xi Zhang³  Yunyi Li³  Hongyou Chen³  Xiaoxian Cui³  Wei Tang³  Chongshan Li³  Yuying Yang³  Songtao Xu⁴  Yan Zhang⁴ 

[1] Chongqing School, University of Chinese Academy of Sciences, Chongqing, China;Department of Immunization Program, Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China;Division of Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China;National Health Commission (NHC) Key Laboratory of Medical Virology and Viral Diseases, Chinese Center for Disease Control and Prevention, Beijing, China;

关键词: measles virus;    complete genome sequences;    Shanghai isolates;    genetic diversity;    vaccine failure;    comparative genomics;   

DOI  :  10.3389/fmed.2022.841650

来源: DOAJ

【 摘 要 】

Although the highly effective measles vaccine has dramatically reduced the incidence of measles, measles, and outbreaks continue to occur in individuals who received the measles vaccine because of immunization failure. In this study, patients who have definite records of immunization were enrolled based on measles surveillance in Shanghai, China, from 2009 to 2017, and genomic characteristics regarding viruses retrieved from these cases provided insights into immunization failure. A total of 147 complete genomes of measles virus (MV) were obtained from the laboratory-confirmed cases through Illumina MiSeq. Epidemiological, and genetic characteristics of the MV were focused on information about age, gender, immunization record, variation, and evolution of the whole genome. Furthermore, systematic genomics using phylogeny and selection pressure approaches were analyzed. Our analysis based on the whole genome of 147 isolates revealed 4 clusters: 2 for the genotype H1 (clusters named H1-A, including 73 isolates; H1-B, including 72 isolates) and the other 2 for D8 and B3, respectively. Estimated nucleotide substitution rates of genotype H1 MV derived using genome and individual genes are lower than other genotypes. Our study contributes to global measles epidemiology and proves that whole-genome sequencing was a useful tool for more refined genomic characterization. The conclusion indicates that vaccination may have an effect on virus evolution. However, no major impact was found on the antigenicity in Shanghai isolates.

【 授权许可】

Unknown