Genetic characterization of novel class 1 Integrons In0, In1069 and In1287 to In1290, and the inference of In1069-associated integron evolution in Enterobacteriaceae

Antimicrobial Resistance and Infection Control

Jiayu Chen¹  Dongguo Wang²  Jianfeng Zhu³  Liman Ma⁴  Kaiyu Zhou⁵  Jiao Feng⁶  Zhe Yin⁶  Dongsheng Zhou⁶ 

[1] Basic Department, Medical College of Shaoxing University;Department of Clinical Laboratory Medicine, Taizhou Municipal Hospital affiliated with Taizhou University and the Institute of Molecular Diagnostics of Taizhou University;Department of Clinical Laboratory Medicine, Yinzhou No. 2 Hospital of Ningbo;Department of Laboratory medicine, Medical College of Taizhou University;Department of the Neurosurgery, Taizhou Municipal Hospital affiliated to Medical College of Taizhou University;State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology;

关键词: Class 1 integron;    Gene cassette;    bla KPC-2;    bla IMP-30;    Enterobacteriaceae;   

DOI  :  10.1186/s13756-017-0241-9

来源: DOAJ

【 摘 要 】

Abstract Background This study aims to characterize genetically related class 1 integrons In1069, In893 and In1287 to In1290, and to further propose a scheme of stepwise integration or excision of individual gene cassettes (GCs) to generation of these integron variations. Methods Six of 139 non-redundant Enterobacteriaceae strains were studied by bacterial antimicrobial susceptibility testing, detection of carbapenemase activity, and integron sequencing and sequence comparison. Results Six novel class 1 integrons, In0, In1069, and In1287 to In1290, together with the previously characterized In893, were determined from the above strains. An unusual bla KPC-2-carrying In0 and the bla IMP-30-carrying In1069 coexists in a single isolate of Escherichia coli. In0 contains a PcH1 promoter and a truncated aacA4’-3 gene cassette (GCaacA4’-3), as well as a bla KPC-2-containing region of Tn6296 integrated between PcH1 and GCaacA4’-3. In1069 carries GCbla IMP-30 and GCaacA4’-3 in this order. The other five integrons, In893 and In1287 to In1290, are genetically related to In1069, and all possess a core GCaacA4’-3. The integration or excision of one or more individual gene cassettes, such as GCbla IMP-30, GCaadA16, GCcatB3, GCarr3 and GCdfrA27, upstream or downstream of GCaacA4’-3 generates various gene cassettes arrays among these five integrons. Conclusions These findings provide the insight into stepwise and parallel evolution of In1069-associated integron variations likely under antibiotic selection pressure in clinical settings.

【 授权许可】

Unknown