Dental pulp stem cell transplantation facilitates neuronal neuroprotection following cerebral ischemic stroke

Biomedicine & Pharmacotherapy

Mingchang Li¹  Qingsong Ye²  Peibang He³  Jianfeng Wang³  Qi Tian³  Yujia Guo³  Pian Gong³  Yan He⁴ 

[1] Corresponding authors.;Center of Regenerative Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China;Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China;Regenerative Medicine Lab, Tianyou Hospital, Wuhan University of Science and Technology, Wuhan, Hubei 430064, China;

关键词: Dental pulp stem cells;    Transplantation;    Ischemic stroke;    Neuroprotection;    Neuronal survival;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objectives: This study aimed to identify and evaluate the intracranial transplantation of dental pulp stem cells (DPSCs) as a possible ischemic stroke therapy that mitigates neuronal death/apoptosis. Materials and methods: DPSCs were isolated from the impacted third molars of healthy volunteers and then intracranially injected at 24 h post-ischemic stroke to Sprague Dawley rats that had been subjected to 2 h of middle cerebral artery occlusion. Neurological functional deficits were assessed using the modified neurological severity score (mNSS), and cerebral edema was quantified using brain water content. Neuronal death/apoptosis was indicated by TdT-mediated dUTP Nick-End Labeling (TUNEL) staining, NeuN immunofluorescence and immunohistochemistry, and Western blot analysis of the protein expression of anti-apoptotic indicator of Bcl-2 and apoptotic indicators of Bax and caspase 3. Results: DPSC transplantation could ameliorate neurological dysfunction and brain edema, reduce infarct volume, decrease the percentage of TUNEL-positive nuclei, increase the number and percentage of NeuN-positive cells in ischemic penumbra, increase the ratio of Bcl-2 and Bax and down-regulate the production of caspase 3 in the cortical infarct zone. Conclusions: DPSC therapy via intracranial injection exerted remarkably neuroprotection mainly by inhibiting neuronal death/apoptosis.

【 授权许可】

Unknown