Biomedicine & Pharmacotherapy | |
Dental pulp stem cell transplantation facilitates neuronal neuroprotection following cerebral ischemic stroke | |
Mingchang Li1  Qingsong Ye2  Peibang He3  Jianfeng Wang3  Qi Tian3  Yujia Guo3  Pian Gong3  Yan He4  | |
[1] Corresponding authors.;Center of Regenerative Medicine, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China;Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China;Regenerative Medicine Lab, Tianyou Hospital, Wuhan University of Science and Technology, Wuhan, Hubei 430064, China; | |
关键词: Dental pulp stem cells; Transplantation; Ischemic stroke; Neuroprotection; Neuronal survival; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Objectives: This study aimed to identify and evaluate the intracranial transplantation of dental pulp stem cells (DPSCs) as a possible ischemic stroke therapy that mitigates neuronal death/apoptosis. Materials and methods: DPSCs were isolated from the impacted third molars of healthy volunteers and then intracranially injected at 24 h post-ischemic stroke to Sprague Dawley rats that had been subjected to 2 h of middle cerebral artery occlusion. Neurological functional deficits were assessed using the modified neurological severity score (mNSS), and cerebral edema was quantified using brain water content. Neuronal death/apoptosis was indicated by TdT-mediated dUTP Nick-End Labeling (TUNEL) staining, NeuN immunofluorescence and immunohistochemistry, and Western blot analysis of the protein expression of anti-apoptotic indicator of Bcl-2 and apoptotic indicators of Bax and caspase 3. Results: DPSC transplantation could ameliorate neurological dysfunction and brain edema, reduce infarct volume, decrease the percentage of TUNEL-positive nuclei, increase the number and percentage of NeuN-positive cells in ischemic penumbra, increase the ratio of Bcl-2 and Bax and down-regulate the production of caspase 3 in the cortical infarct zone. Conclusions: DPSC therapy via intracranial injection exerted remarkably neuroprotection mainly by inhibiting neuronal death/apoptosis.
【 授权许可】
Unknown