The Study of Angptl4-Modulated Podocyte Injury in IgA Nephropathy

Frontiers in Physiology

Sha Jia²  Ying Zhang⁴  Yucheng Wang⁴  Jianghua Chen⁴  Hong Jiang⁴  Xiaofeng Peng⁴  Qin Zhou⁴  Ludan Liang⁴  Pingping Ren⁴  Shi Feng⁴  Cuili Wang⁴  Meng Li⁴  Xiujin Shen⁴ 

[1] Children Hospital, Dongyang, China;;Dongyang Women &Institute of Nephropathy, Zhejiang University, Hangzhou, China;Key Laboratory of Multiple Organ Transplantation, Ministry of Health of China, Beijing, China;Key Laboratory of Nephropathy, Hangzhou, China;Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China;Kidney Disease Immunology Laboratory, The Third-Grade Laboratory, State Administration of Traditional Chinese Medicine of China, Hangzhou, China;

关键词: Angptl4;    podocyte;    immunoglobulin A nephropathy;    RNA-Seq;    progression;   

DOI  :  10.3389/fphys.2020.575722

来源: DOAJ

【 摘 要 】

BackgroundIncreasing evidence shows that Angptl4 affects proteinuria in podocytes injured kidney disease, however, whether there is a relationship between Angptl4 and IgA nephropathy (IgAN) has not been studied yet.MethodsPlasma and urine samples were obtained from 71 patients with IgAN and 61 healthy controls. Glomeruli from six renal biopsy specimens (three IgAN patients and three healthy controls) were separated by RNA-Seq. Differentially expressed genes (DEGs) related to podocytes and Angptl4 between IgAN patients and healthy controls were performed using the Limma package. Gene set enrichment analysis was used to determine whether there was a statistically significant difference between the two groups. STRING was used to create a protein-protein interaction network of DEGs. Association analysis between Angptl4 levels and clinical features of IgAN was performed.ResultsThirty-three podocyte-related and twenty-three Angpt4-related DEGs were found between IgAN patients and healthy controls. By overlapping the genes, FOS and G6PC were found to be upregulated in IgAN patients, while MMP9 was downregulated in IgAN patients. Plasma and urine Angptl4 levels were closely related to the degree of podocyte injury and urine protein, but not to the protein-creatine ratio.ConclusionOur findings show that Angptl4 levels in plasma and urine are related to podocyte damage and, therefore, may be a promising tool for assessing the severity of IgAN patients to identify and reverse the progression to ESRD.

【 授权许可】

Unknown