LncPrep + 96kb 2.2 kb Inhibits Estradiol Secretion From Granulosa Cells by Inducing EDF1 Translocation

Frontiers in Cell and Developmental Biology

Hongdan Zhang¹  Li Xiao¹  Weihui Wen¹  Fen Feng¹  Suo Han¹  Chunping Zhang¹  Xiating Tong¹  Jing Wang²  Long Li³  Riqiang Bao³ 

[1] Department of Cell Biology, College of Medicine, Nanchang University, Nanchang, China;Department of Microbiology, College of Medicine, Nanchang University, Nanchang, China;Joint Program of Nanchang University and Queen Mary University of London, College of Medicine, Nanchang University, Nanchang, China;

关键词: estradiol;    lncPrep + 96kb;    EDF1;    aromatase;    granulosa cell;   

DOI  :  10.3389/fcell.2020.00481

来源: DOAJ

【 摘 要 】

LncPrep + 96kb is a novel long non-coding RNA expressed in murine granulosa cells with two transcripts that are 2.2 and 2.8 kb in length. However, the potential roles of lncPrep + 96kb in granulosa cells remain poorly understood. In this study, we investigated the effect of the lncPrep + 96kb 2.2 kb transcript on granulosa cells through the overexpression and knockdown of lncPrep + 96kb 2.2 kb. We found that lncPrep + 96kb 2.2 kb inhibited aromatase expression and estradiol production. Endothelial differentiation-related factor 1 (EDF1) is an evolutionarily conserved transcriptional coactivator. We found that EDF1 knockdown inhibited aromatase expression and estradiol production. The RNA immunoprecipitation results also showed that lncPrep + 96kb 2.2 kb can bind to EDF1 and that overexpression of lncPrep + 96kb 2.2 kb induced the translocation of EDF1 from the nucleus to the cytoplasm. The CatRAPID signature revealed that the 1,979–2,077 and 603–690 nucleotide positions in lncPrep + 96kb 2.2 kb were potential binding sites for EDF1. We found that mutating the 1,979–2,077 site rescued the effects of lncPrep + 96kb 2.2 kb on aromatase expression and estradiol production. In conclusion, we are the first to report that specific expression of lncPrep + 96kb 2.2 kb in granulosa cells inhibits the production of estradiol by influencing the localization of EDF1 in granulosa cells. The 1,979–2,077 site of lncPrep + 96kb 2.2 kb contributes to the ability to bind to EDF1.

【 授权许可】

Unknown