Candida parapsilosis Biofilm Identification by Raman Spectroscopy

International Journal of Molecular Sciences

Martina Mahelová¹  Katarina Mlynariková¹  Filip Růžička¹  Veronika Holá¹  Jan Ježek²  Martin Šiler²  Silvie Bernatová²  Vladislav Krzyžánek²  Pavel Zemánek²  Ota Samek² 

[1] Department of Microbiology, Faculty of Medicine and St. Anne's Faculty Hospital, Brno 65691, Czech Republic;Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic, v.v.i., Královopolská 147, Brno 61264, Czech Republic;

关键词: Raman spectroscopy;    Candida parapsilosis;    biofilm;   

DOI  :  10.3390/ijms151223924

来源: DOAJ

【 摘 要 】

Colonies of Candida parapsilosis on culture plates were probed directly in situ using Raman spectroscopy for rapid identification of specific strains separated by a given time intervals (up to months apart). To classify the Raman spectra, data analysis was performed using the approach of principal component analysis (PCA). The analysis of the data sets generated during the scans of individual colonies reveals that despite the inhomogeneity of the biological samples unambiguous associations to individual strains (two biofilm-positive and two biofilm-negative) could be made.

【 授权许可】

Unknown