Design and Synthesis of Multi-Functional Superparamagnetic Core-Gold Shell Coated with Chitosan and Folate Nanoparticles for Targeted Antitumor Therapy

Nanomaterials

Salim Albukhaty¹  Hassan Al-Karagoly²  Sharafaldin Al-Musawi³  Faizah Almalki⁴ 

[1] Department of Chemistry, College of Science, University of Misan, Maysan 62001, Iraq;Department of Internal and Preventive Medicine, Veterinary Medicine College, University of Al-Qadisiyah, Al-Diwaniyah 58002, Iraq;Faculty of Biotechnology, Al-Qasim Green University, Babylon 51013, Iraq;Faculty of Science, Taif University, Taif 21944, Saudi Arabia;

关键词: superparamagnetic iron oxide nanoparticles;    gold nanoparticles;    doxorubicin;    chitosan;    breast cancer;    folate;   

DOI  :  10.3390/nano11010032

来源: DOAJ

【 摘 要 】

A dual-targeting nanomedicine composed of pH-sensitive superparamagnetic iron oxide core-gold shell SPION@Au, chitosan (CS), and folate (FA) was developed as a doxorubicin (DOX) antitumor medication. Microemulsion was used for preparation and cross-linking conjugation. The characteristics of the designed nanocomposite were studied using atomic force microscopy (AFM), transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction, UV-visible spectroscopy, Zeta potential and vibrating sample magnetometry (VSM), and Fourier transform infrared spectroscopy. The prepared SPION@Au-CS-DOX-FA nanoparticles (NPs) were spherical with an average diameter of 102.6 ± 7 nm and displayed an elevated drug loading behavior and sustained drug release capacity. The SPION@Au-CS-DOX-FA NPs revealed long term anti-cancer efficacy due to their cytotoxic effect and apoptotic inducing efficiency in SkBr3 cell lines. Additionally, Real-time PCR outcomes significantly showed an increase in BAK and BAX expression and a decrease in BCL-XL and BCL-2. In vivo results revealed that SPION@Au significantly decreased the tumor size in treated mice through magnetization. In conclusion, prepared SPION@Au-CS-DOX-FA could be a beneficial drug formulation for clinical breast cancer treatment.

【 授权许可】

Unknown