Evaluation of protein extraction methods to obtain protein concentrate from cassava leaf

Engenharia Agrícola

Anderson Coldebella¹  Marney P. Cereda²  Priscila F. Coldebella³  Simone D. Gomes⁴  Sílvia R. M. Coelho⁴  Janete A. Evarini⁴ 

[1] Instituto Federal do Paraná;Universidade Católica Dom Bosco;Universidade Estadual de Maringá;Universidade Estadual do Oeste do Paraná;

关键词: exploitation;    agricultural waste;    protein extraction;   

DOI  :  10.1590/S0100-69162013000600015

来源: DOAJ

【 摘 要 】

The cassava leaf, waste generated in the harvest of the roots, is characterized by high content of protein, vitamins and minerals; however, its use is limited due to the high fiber content and antinutritional substances, which can be removed by obtaining protein concentrates. In this context, the objective of this study was to evaluate protein extraction processes, aiming the use of cassava leaves (Manihot esculenta Crantz) as an alternative protein. Four methods were tested: 1) Coagulation of Proteins by Lowering the Temperature, 2) Extraction by Isoelectric Precipitation, 3) Solubilization of Proteins and 4) Fermentation of Filter Leaf Juice. To obtain the concentrates, the use of fresh or dried leaves and extraction in one or two steps were also evaluated. The solubilization of proteins (method 3) showed a higher extraction yield; however, with concentrate of low quality. The fermentation of the juice (method 4) produced concentrates with higher quality and lower costs and the isoelectric precipitation (method 2) promoted the obtention of concentrates in less time, both with good prospects for use. The use of two extraction steps was not advantageous to the process and there was no difference between the use of fresh or dried leaf, and the use of fresh leaves is presented as a good option for the simplicity of the method.

【 授权许可】

Unknown