Evaluation of the NLRP3 Inflammasome Activating Effects of a Large Panel of TiO2 Nanomaterials in Macrophages

Nanomaterials

RoelP. F. Schins¹  Julia Kolling¹  Jonas Tigges¹  Catrin Albrecht¹  Bryan Hellack² 

[1] IUF—Leibniz Research Institute for Environmental Medicine, Auf’m Hennekamp 50, 40225 DE Düsseldorf, Germany;Institute of Energy and Environmental Technology e.V. (IUTA), Bliersheimer Str. 60, 47229 Duisburg, Germany;

关键词: nanomaterials;    titanium dioxide;    NALP3;    interleukin-1beta;    NR8383;    bone marrow derived macrophages;   

DOI  :  10.3390/nano10091876

来源: DOAJ

【 摘 要 】

TiO₂ nanomaterials are among the most commonly produced and used engineered nanomaterials (NMs) in the world. There is controversy regarding their ability to induce inflammation-mediated lung injuries following inhalation exposure. Activation of the NACHT, LRR and PYD domains-containing protein 3 (NALP3) inflammasome and subsequent release of the cytokine interleukin (IL)-1β in pulmonary macrophages has been postulated as an essential pathway for the inflammatory and associated tissue-remodeling effects of toxic particles. Our study aim was to determine and rank the IL-1β activating properties of TiO₂ NMs by comparing a large panel of different samples against each other as well as against fine TiO₂, synthetic amorphous silica and crystalline silica (DQ12 quartz). Effects were evaluated in primary bone marrow derived macrophages (BMDMs) from NALP3-deficient and proficient mice as well as in the rat alveolar macrophage cell line NR8383. Our results show that specific TiO₂ NMs can activate the inflammasome in macrophages albeit with a markedly lower potency than amorphous SiO₂ and quartz. The heterogeneity in IL-1β release observed in our study among 19 different TiO₂ NMs underscores the relevance of case-by-case evaluation of nanomaterials of similar chemical composition. Our findings also further promote the NR8383 cell line as a promising in vitro tool for the assessment of the inflammatory and inflammasome activating properties of NMs.

【 授权许可】

Unknown