A structure-supporting, self-healing, and high permeating hydrogel bioink for establishment of diverse homogeneous tissue-like constructs

Bioactive Materials

Jing Zhang¹  Fei Fei²  Libiao Liu³  Dezhi Zhou⁴  Tao Xu⁴  Zhenguo Nie⁵  Zhou Fei⁵  Hongqing Chen⁶  Xinda Li⁷  Haitao Zhang⁸ 

[1] Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, 610072, China;Department of Neurosurgery, Central Theater General Hospital, Wuhan, 430010, People's Republic of China;Department of Neurosurgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China;Key Laboratory for Advanced Materials Processing Technology, Ministry of Education, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China;Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, People's Republic of China;Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, People's Republic of China;Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, People's Republic of China;Department of Orthopedics, Fourth Medical Center of PLA General Hospital, 100048, Beijing, People's Republic of China;

关键词: Time-sharing structure-supporting;    High permeability;    Bioink;    Homogeneous cell growth;    Tissue-like constructs;   

DOI  :  

来源: DOAJ

【 摘 要 】

The ready-to-use, structure-supporting hydrogel bioink can shorten the time for ink preparation, ensure cell dispersion, and maintain the preset shape/microstructure without additional assistance during printing. Meanwhile, ink with high permeability might facilitate uniform cell growth in biological constructs, which is beneficial to homogeneous tissue repair. Unfortunately, current bioinks are hard to meet these requirements simultaneously in a simple way. Here, based on the fast dynamic crosslinking of aldehyde hyaluronic acid (AHA)/N-carboxymethyl chitosan (CMC) and the slow stable crosslinking of gelatin (GEL)/4-arm poly(ethylene glycol) succinimidyl glutarate (PEG-SG), we present a time-sharing structure-supporting (TSHSP) hydrogel bioink with high permeability, containing 1% AHA, 0.75% CMC, 1% GEL and 0.5% PEG-SG. The TSHSP hydrogel can facilitate printing with proper viscoelastic property and self-healing behavior. By crosslinking with 4% PEG-SG for only 3 min, the integrity of the cell-laden construct can last for 21 days due to the stable internal and external GEL/PEG-SG networks, and cells manifested long-term viability and spreading morphology. Nerve-like, muscle-like, and cartilage-like in vitro constructs exhibited homogeneous cell growth and remarkable biological specificities. This work provides not only a convenient and practical bioink for tissue engineering, targeted cell therapy, but also a new direction for hydrogel bioink development.

【 授权许可】

Unknown