Technical Aspects of Coenzyme Q10 Analysis: Validation of a New HPLC-ED Method

Antioxidants

Pedro Ruiz-Sala¹  Patricia Alcaide¹  Clara Oliva²  Raquel Montero²  Rafael Artuch²  George J. G. Ruijter³  Judit García-Villoria⁴  Abraham J. Paredes-Fuentes⁴ 

[1] Centro de Diagnóstico de Enfermedades Moleculares, Universidad Autónoma Madrid, IdiPAZ, 28049 Madrid, Spain;Clinical Biochemistry Department, Institut de Recerca Sant Joan de Déu, Hospital Sant Joan de Déu, Esplugues de Llobregat, 08950 Barcelona, Spain;Department of Clinical Genetics, Center for Lysosomal and Metabolic Diseases, Erasmus University Medical Center, 3015 GD Rotterdam, The Netherlands;Division of Inborn Errors of Metabolism-IBC, Biochemistry and Molecular Genetics Department, Hospital Clínic de Barcelona, 08028 Barcelona, Spain;

关键词: coenzyme Q₁₀;    HPLC with electrochemical detection;    electrochemical cells;    external quality control;    ERNDIM;   

DOI  :  10.3390/antiox11030528

来源: DOAJ

【 摘 要 】

The biochemical measurement of the CoQ status in different tissues can be performed using HPLC with electrochemical detection (ED). Because the production of the electrochemical cells used with the Coulochem series detectors was discontinued, we aimed to standardize a new HPLC-ED method with new equipment. We report all technical aspects, troubleshooting and its performance in different biological samples, including plasma, skeletal muscle homogenates, urine and cultured skin fibroblasts. Analytical variables (intra- and inter-assay precision, linearity, analytical measurement range, limit of quantification, limit of detection and accuracy) were validated in calibrators and plasma samples and displayed adequate results. The comparison of the results of a new ERNDIM external quality control (EQC) scheme for the plasma CoQ determination between HPLC-ED (Lab 1) and LC-MS/MS (Lab 2) methods shows that the results of the latter were slightly higher in most cases, although a good consistency was generally observed. In conclusion, the new method reported here showed a good analytical performance. The global quality of the EQC scheme results among different participants can be improved with the contribution of more laboratories.

【 授权许可】

Unknown