Significant association between Taq1 gene polymorphism in vitamin D receptor and chronic spontaneous urticaria in the Northeast of Iran

Clinical and Molecular Allergy

Farahzad Jabbari-Azad¹  Hanieh Zamani¹  Maryam Khoshkhui¹  Farzaneh Iravani²  Jalil Tavakkol-Afshari³  Mojgan Mohammadi³  Maryam Davarpanah³  Alireza Hamidian Jahromi⁴  Hadi Zare Marzouni⁵ 

[1] Allergy Research Center, Mashhad University of Medical Sciences;Genetic Research Center, Mashhad University of Medical Sciences;Immunology Research Center, Mashhad University of Medical Sciences;Plastic and Reconstructive Surgery Department, Rush University Medical Center;Qaen School of Nursing and Midwifery, Birjand University of Medical Sciences;

关键词: Taq1 gene polymorphism;    Vitamin D receptor;    Chronic spontaneous urticaria;    Allergy;    CSU;    VDR gene;   

DOI  :  10.1186/s12948-021-00145-w

来源: DOAJ

【 摘 要 】

Abstract Objective Chronic spontaneous urticaria (CSU) is defined as urticaria with an unknown etiology which persists for more than 6 weeks. CSU is an uncomfortable cutaneous condition that occurs due to an immune-mediated inflammatory reaction. Many studies have demonstrated that vitamin D deficiency and single-nucleotide polymorphisms in the vitamin D receptor (VDR) impact the immune response. In the current study, the frequency of the Taq1 polymorphism in the VDR gene were compared between patients with CSU and individuals without CSU. Methods In a case–control study, a group of CSU patients (n = 100) was compared with a group of healthy age- and gender-matched individuals as a control group (n =100) who visited our center between 2015 and 2017. After DNA extraction from EDTA-containing blood, polymerase chain reaction (PCR–RFLP) was used to determine the presence of the Taq1 polymorphism. Serum vitamin D levels were measured using ELISA method (Abcam, Cambridge, USA). Results Genotyping for Taq1 polymorphism showed that TT, Tt and tt genes frequency in the CSU group were 36%, 54%, and 10% respectively. The TT, Tt and tt genotypes had a distribution of 50%, 47% and 3% respectively in the control group. The mean serum vitamin D level in the CSU group was 19.88 ± 8.14 ng/ml, which was not significantly correlated with the Taq1 polymorphism (P = 0.841). There was a significant relationship between Taq1 gene polymorphism (tt genotype) and CSU (P = 0.038). Tt genotype increased the risk of CSU (odds ratio = 1.596), and inheritance of tt genotype increased the risk even further (odds ratio = 4.630). Conclusion The frequency of Taq1 genotype polymorphism in the VDR gene was significantly higher in patients with CSU compared to the control group. The tt genotype polymorphism may be a risk factor for CSU.

【 授权许可】

Unknown