Integrated Metabolomics and Proteomics Analysis of Urine in a Mouse Model of Posttraumatic Stress Disorder

Frontiers in Neuroscience

Fei Li¹  Feng Pan¹  Yi Huang¹  Daxue Zhou¹  Zihan Zheng¹  Yan Shao¹  Wei Sun¹  Yiwei Huang¹  Xiaoyang Wang¹  Chengyan Long²  Yanlei Guo²  Gang Chen³ 

[1] Biomedical Analysis Center, Army Medical University, Chongqing, China;Chongqing Academy of Chinese Materia Medica, Chongqing, China;Chongqing Key Laboratory of Cytomics, Chongqing, China;Key Laboratory of Extreme Environmental Medicine, Ministry of Education of China, Chongqing, China;

关键词: posttraumatic stress disorder (PTSD);    PTSD model;    urine;    metabolomics;    proteomics;    mice;   

DOI  :  10.3389/fnins.2022.828382

来源: DOAJ

【 摘 要 】

Posttraumatic stress disorder (PTSD) is a serious stress disorder that occurs in individuals who have experienced major traumatic events. The underlying pathological mechanisms of PTSD are complex, and the related predisposing factors are still not fully understood. In this study, label-free quantitative proteomics and untargeted metabolomics were used to comprehensively characterize changes in a PTSD mice model. Differential expression analysis showed that 12 metabolites and 27 proteins were significantly differentially expressed between the two groups. Bioinformatics analysis revealed that the differentiated proteins were mostly enriched in: small molecule binding, transporter activity, extracellular region, extracellular space, endopeptidase activity, zymogen activation, hydrolase activity, proteolysis, peptidase activity, sodium channel regulator activity. The differentially expressed metabolites were mainly enriched in Pyrimidine metabolism, D-Glutamine and D-glutamate metabolism, Alanine, aspartate and glutamate metabolism, Arginine biosynthesis, Glutathione metabolism, Arginine, and proline metabolism. These results expand the existing understanding of the molecular basis of the pathogenesis and progression of PTSD, and also suggest a new direction for potential therapeutic targets of PTSD. Therefore, the combination of urine proteomics and metabolomics explores a new approach for the study of the underlying pathological mechanisms of PTSD.

【 授权许可】

Unknown