Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation, Biofilm Formation, and Breast Cancer

Marine Drugs

Fatma Elleuch¹  Chantal Pichon¹  Nesrine Gargouch²  Christine Gardarin²  Céline Laroche²  Imen Fendri³  Ines Karkouch⁴  Olfa Tabbene⁴  Luc Picton⁵  Christophe Rihouey⁵  Slim Abdelkafi⁶ 

[1] Centre de Biophysique Moléculaire, CNRS-UPR 4301, 45071 Orléans, France;Institut Pascal, CNRS, SIGMA Clermont, Université Clermont Auvergne, F-63000 Clermont-Ferrand, France;Laboratoire de Biotechnologie Végétale Appliquée à l’Amélioration des Cultures, Faculty of Sciences of Sfax, University of Sfax, Sfax 3000, Tunisia;Laboratory of Bioactive Substances, Biotechnology Center of Borj-Cedria (CBBC), BP-901, Hammam-Lif 2050, Tunisia;Normandie University, UNIROUEN, INSA Rouen, CNRS, PBS, 76000 Rouen, France;Unité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of Sfax, Sfax 3038, Tunisia;

关键词: Porphyridium marinum;    exopolysaccharide;    high pressure homogenizer;    antibacterial activity;    anti-biofilm activity;    anti-cancer activity;   

DOI  :  10.3390/md19020066

来源: DOAJ

【 摘 要 】

Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs. However, lot of them remain to be discovered and tested. In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells. Results have shown that all EPS samples present some biological activity. For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain. For Candida albicans, the biofilm formation was reduced by about 90% by using only a 31.3 µg/mL concentration. Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%. Finally, analyses of polymers composition and viscosity measurements were conducted on all samples, in order to propose hypotheses involving the activities caused by the intrinsic properties of polymers.

【 授权许可】

Unknown