Bioactivities of fish protein hydrolysates from defatted salmon backbones

Biotechnology Reports

Peter Eck¹  Kathrine Five²  Turid Rustad²  Revilija Mozuraityte³  Rasa Slizyte³  Katariina Rommi⁴ 

[1] Department of Human Nutritional Sciences, University of Manitoba, Canada;Norwegian University of Science and Technology, Norway;SINTEF Fisheries and Aquaculture, Norway;VTT Technical Research Centre of Finland Ltd, Finland;

关键词: Fish protein hydrolysates;    Salmon backbones;    Antioxidant;    ACE-inhibition;    Glucose transport inhibition;   

DOI  :  10.1016/j.btre.2016.08.003

来源: DOAJ

【 摘 要 】

Bioactivities of bulk fish protein hydrolysates (FPH) from defatted salmon backbones obtained with eight different commercial enzymes and their combinations were tested. All FPH showed antioxidative activity in vitro. DPPH scavenging activity increased, while iron chelating ability decreased with increasing time of hydrolysis. All FPH showed ACE inhibiting effect which depended on type of enzyme and increased with time of hydrolysis. The highest effect was found for FPH produced with Trypsin. Bromelain + Papain hydrolysates reduced the uptake of radiolabelled glucose into CaCo-2 cells, a model of human enterocytes, indicating a potential antidiabetic effect of FPH. FPH obtained by Trypsin, Bromelain + Papain and Protamex showed the highest ACE inhibitory, cellular glucose transporter (GLUT/SGLT) inhibitory and in vitro antioxidative activities, respectively. Correlation was observed between the measured bioactivities, degree of hydrolysis and molecular weight profiles, supporting prolonged hydrolysis to obtain high bioactivities.

【 授权许可】

Unknown