Evaluation of criteria for diagnosis of atopic dermatitis and detection of allergen specific IgE antibodies in dogs allergic to Ambrosia artemisiifolia pollen

Acta Veterinaria

关键词: Ambrosia artemisiifolia;    canine atopic dermatitis;    immunoglobulin E (IgE);    ragweed pollen;   

DOI  :  10.2298/AVB1304437M

来源: DOAJ

【 摘 要 】

Common ragweed (Ambrosia atremisiifolia) is one of the most frequent causesof pollen-induced allergic reactions both in humans and dogs. It has not beendefined yet, what is the major allergen(s) to which most dogs allergic toragweed show a positive result on intradermal skin test (IDST). In thepresent study sensitization to Ambrosia artemisiifolia pollen allergens indogs with atopic dermatitis was examined with both in vivo and in vitrotests, including IDST and serum allergen specific IgE test. Detection ofspecific-IgE antibodies against ragweed allergens by immunoblotting in thesera of allergic dogs was optimized, as well. Dogs that were positive, asjudged by IDST reactions to ragweed pollen allergens, also had alergenspecific IgE antibodies in their sera. Results indicate that major allergensof A. artemisifolia pollen in dogs are Amb a 1 and Amb a 2. Furthercharacterization of ragweed allergens is needed before they could potentiallybe used in intradermal testing or allergen immunotherapy in affected dogs.Also, we evaluated new Favrots diagnostic criteria for canine atopicdermatitis in dogs allergic to Ambrosia atremisiifolia pollen. It might beconcluded that proposed criteria are of great assistance for seting upsuspected diagnosis of canine atopic dermatitis, after ruling out otherpruritic dermatoses. [Projekat Ministarstva nauke Republike Srbije, br.172024]

【 授权许可】

Unknown